Enspire Formula Samples: A Comprehensive Analysis

Lipidomic analysis has identified a total of 393 lipid species across both positive and negative ionization modes, with the major classes detected being triglycerides, sphingomyelins, and several phospholipids. In addition to these lipid species, the analysis also revealed that the major protein composition varied significantly across different MFGM fractions, with an abundance of milk (casein and whey) proteins present in all samples.

The proteomic analysis using bottom-up data-dependent liquid chromatography–tandem mass spectrometry (LC-MS/MS) identified nearly a thousand proteins across all samples, with 364 of them exhibiting different abundance levels across the tested samples. This suggests significant differences in protein composition among the MFGM fractions.

Furthermore, the MFGM samples were compared to two infant formulas, including a standard formula and a premium formula containing MFGM. Six bovine milk fractions were obtained from various commercial sources, representing MFGM fractions or phospholipid fractions. The aim of this study was to characterize the composition of commercial MFGM samples through a combination of proteomic and lipidomic analyses.

As MFGM is a heterogeneous mixture of fat, protein, and carbohydrate, it can be expected that variations among MFGM products exist. Commercial bovine sources have recently emerged as a novel food ingredient and have been added to various products, including infant formula. The characterization of MFGM composition through this study provides valuable insights into the differences between commercial MFGM samples.

In conclusion, this comprehensive analysis has identified 393 lipid species and nearly a thousand proteins across all MFGM fractions, with significant differences in protein composition among the fractions. The comparison to two infant formulas and six bovine milk fractions highlights the importance of understanding the composition of commercial MFGM samples. This study demonstrates the value of combining proteomic and lipidomic analyses to characterize the complex composition of MFGM, providing valuable insights for manufacturers and consumers alike.

Enspire formula samples